Lipid Mixing Method
The procedure described here can be used to supplement monoolein or any other LCP host lipid with lipophilic additives. Such additives can modulate properties of LCP or can preferentially interact with proteins reconstituted in LCP and therefore are useful for optimization of crystallization conditions. Cholesterol was found to be the best lipid additive significantly improving the β2AR-T4L crystal size (Cherezov et al., 2007; Hanson et al., 2008). Cholesterol was also used for crystallization of adenosine A2A receptor (Jaakola et al., 2008). Compatibility of monoolein-based LCP with several lipid-like molecules was reported in Cherezov et al., 2002.
For additional details see Caffrey and Cherezov, 2009.
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