Orphanin FQ Receptor

NAME:Nociceptin/Orphanin FQ Receptor

PDB IDs: 4EA3

SOURCE ORGANISM: Homo sapiens

SEQUENCE: OPRX_HUMAN>> , C562_ECOLX>>

ORIENTATION IN MEMBRANE: OPM >>

GPCRDB: OPRX_HUMAN >>

CRYSTALLIZATION CONDITIONS:

LCP 40 nL

Ppt solution 0.8 µL

Lipid 60 %w/w	Protein Solution 40 %w/w
Lipid 1: 90 %w/w monoolein	Protein: 40 mg/mL NOP	Buffer: 100 mM Bis-Tris propane pH 6.4
Lipid 2: 10% w/w Cholesterol	Buffer: 50 mM HEPES pH 7.5	Precipitant: 25-30 %v/v PEG 400
	Detergent1: 0.025% DDM	Salt: 100-200 mM K/Na tartrate
	Detergent2 0.005% CHS
	Ligand: 100 μM Compound-24
	Salt: 500 mM NaCl, 20 mM KCl

Temperature (°C): 20 Crystal growth time (d): 14 Crystal size (µm): 40 x 10 x 3

References: Thompson, A.A., Liu, W., Chun, E., Katritch, V., Wu, H., Vardy, E., Huang, X.-P., Trapella, C., Guerrini, R., Calo, G., Roth, B.L., Cherezov, V., Stevens, R.C. (2012) Structure of the nociceptin/orphanin FQ receptor in complex with a peptide mimetic Nature

SPACE GROUP: P 21 UNIT CELL: 42.11 170.94 65.35 90 103.14 90

CRYSTAL PACKING: