M3 Muscarinic Acetylcholine Receptor

NAME:M3 Muscarinic Acetylcholine Receptor

PDB IDs: 4DAJ

SOURCE ORGANISM: Rattus norvegicus

SEQUENCE: ACM3_RAT>> , LYS_BPT4>>

ORIENTATION IN MEMBRANE: OPM >>

GPCRDB: ACM3_RAT >>

CRYSTALLIZATION CONDITIONS:

LCP 15 nL

Ppt solution 0.6 µL

Lipid 60 %w/w	Protein Solution 40 %w/w
Lipid 1: 90 %w/w monoolein	Protein: 60 mg/mL M2	Buffer: 100 mM Hepes pH 7.5
Lipid 2: 10% w/w Cholesterol	Buffer: 20 mM HEPES pH 7.5	Precipitant: 27-38 %v/v PEG 400
	Detergent1: 0.01% MNG	Salt: 100 mM NH4 phosphate
	Detergent2 0.001% CHS	Additive: 1 % 1,2,3-heptanetriol
	Ligand: 10 μM Tiotropium
	Salt: 100 mM NaCl

Temperature (°C): 20 Crystal growth time (d): 2-3 Crystal size (µm):

References: Kruse, A.C., Hu, J., Pan, A.C., Arlow, D.H., Rosenbaum, D.M., Rosemond, E., Green, H.F., Liu, T., Chae, P.S., Dror, R.O., Shaw, D.E., Weis, W.I., Wess, J., Kobilka, B.K. (2012) Structure and dynamics of the M3 muscarinic acetylcholine receptor Nature 482: 552-556 >>

SPACE GROUP: P 1 UNIT CELL: 54.77 61.31 176.91 85.87 89.90 84.90

CRYSTAL PACKING: