Structures from LCP >> Dopamine D3 receptor bound to eticlopride
NAME:Dopamine D3 receptor
PDB IDs: 3PBL
SOURCE ORGANISM: Homo sapiens
SEQUENCE: UniProt P35462>>
ORIENTATION IN MEMBRANE: OPM >>
GPCRDB: DRD3_HUMAN >>
CRYSTALLIZATION CONDITIONS:
| LCP 50 nL | Ppt solution 0.8 µL |
| Lipid 60 %w/w | Protein Solution 40 %w/w | |
| Lipid 1: 90 %w/w Monoolein | Protein: 30 mg/mL D3R-T4L | Buffer: 100 mM bis-tris propane pH 7.5 |
| Lipid 2: 10 %w/w Cholesterol | Ligand: 1 mM eticlopride | Precipitant: 30 %v/v PEG 400 |
| Buffer: 25 mM Hepes pH 7.5 | Salt: 0.3 M Ammonium Acetate | |
| Detergent 1: 0.05 %w/v DDM | Ligand: 1 mM eticlopride | |
| Detergent 2: 0.01%w/v CHS | Additive: 2% glucose | Salt: 500 mM NaCl |
Temperature (°C): 20 Crystal growth time (d): 14 Crystal size (µm): 40 x 20 x 10
References: E. Y. Chien, W. Liu, Q. Zhao, V. Katritch, G.W. Han, M.A. Hanson, L. Shi, A.H. Newman, J.A. Javitch, V. Cherezov and R. C. Stevens (2010) Structure of the Human Dopamine D3 Receptor in Complex with a D2/D3 Selective Antagonist Science 330: 1091-1095 >>
SPACE GROUP: P 21 21 21 UNIT CELL: 88.83 92.49 176.12 90 90 90
CRYSTAL PACKING:
